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An aliquot of serum was added to methanol. The mixture was vortex and centrifuged. The supernatant was transferred into clean tube with Al. The mixture was vortex. Then add H3PO4 and CCL3. After the mixture was vortexed and centrifuged for one minute at 2500 rpm, the chloroform extract was evaporated and the residue was redissolved methanol. Sample was injected into high performance liquid chromatography (HPLC).

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