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You selected inventions in category Life Sciences

  • Peptide Tag for Stabilizing Protein in the CellPeptide Tag for Stabilizing Protein in the Cell
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      Description

      The proposed technology is a protein motif that enables the stabilization of proteins in the cell. The motif is composed of 16 amino acids mainly consisting of acidic amino acids. This motif provides proteolysis resistance to the fused protein, so that the expression level is highly improved by approximately 2-20 times. By employing this motif as a tag of the expression plasmid, it is possible to provide an expression system that can raises the expression of proteins which are normally expressed at a low level. Furthermore, as the other application, applying this motif to cell-permeable proteins used for protein therapies would result in the improvement of the effectiveness of the therapies, since the protein therapies generally face the problems of the protein degradation.

      Features and Advantages
      * Motif derived from transcription factor DP-1, and named as DP-1 Stabilon
      - Provides proteolysis resistance by inhibiting proteasome and serine protease
      - Composed of 16 amino acids mainly consisting of acidic ones (EDDEEDDDFNENDEDD)
      - Can be shortened to 5 acidic amino acids (EDDEE)
      - Has effect in both positions of N-terminus and C-terminus to be selected
      * The expression of many proteins increased (approx. 2-20 times)
      - eg. Sox2, PARalpha, Foxp3, Oct4, Klf4, Glis1, NFYC, HSPA14 and RhoA
      * PCT application filed

      Possible Applications
      * Tag for plasmid systems to improve protein expression
      * Stabilization of therapeutic proteins, therapeutic antibodies

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    • Novel Mouse Model for Rheumatoid ArthritisNovel Mouse Model for Rheumatoid Arthritis
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        Provided is a new animal model for human rheumatoid arthritis (RA), which is produced by inoculating Epstein-Barr virus (EBV). A number of observations have suggested the involvement of EBV in the pathogenesis of RA. However, there have been no appropriate animal models for EBV infection to examine a causal relationship between EBV and RA, because EBV can infect only limited primate species and does not infect normal mice. By employing the NOD/Shi-scid/IL-2Rynull (NOG) mouse strain, the inventor has successfully developed humanized mouse model of EBV infection. This newly developed animal model shows erosive arthritis with many features resembling those of RA so that be useful for pathological elucidation and drug evaluation of RA. Furthermore, this animal model has advantages over conventional RA animal models in that it can be produced easily with low cost and suitable for examining development and progress of inflammation.

        Features and Advantages
        * Human immune system components are reconstituted in NOG mice
        - CD34+ hematopoietic stem cells isolated from cord blood is transplanted in NOG mice
        * Erosive arthritis is accompanied by many features resembling RA
        - Pannus formation, synovial membrane proliferation,
        - Inflammatory cell infiltration to the synovium, and bone marrow edema
        * Slow development and progression of inflammation like those of human RA
        - Suitable for pathological elucidation and drug evaluation
        * Simple and low-cost production method
        * PCT application filed

        Possible Applications
        * Animal experiment for drug development and pathological elucidation of RA

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      • Bone marrow-directing drug delivery materialsBone marrow-directing drug delivery materials
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          Description

          The bone marrow play an important role as a hematopoietic organ, and bone diseases such as osteomyelitis and myeloma cause severe morbidity. Since the bone marrow is not an organ to which surgical therapy is an option, the bone marrow diseases are mainly subject to medical treatment, such as by chemotherapy. There is a great need for drug delivery systems that have the ability to effectively deliver therapeutic agents to the bones or bone marrows. The inventors have identified novel drug delivery materials that have the ability to target therapeutic agents to the bones with high specificity. These agents also have the ability to result in accumulation of therapeutic agents in bones or bone marrow. This is based on the fact that the vesicles were intravenously administered to a living body and the distribution of the vesicles in the organs in the body was quantitatively analyzed. The experiments have been conducted using rabbit and monkeys.

          Features and Advantages:
          * Nano-sized lipid vesicles (liposome technology)
          * Surface modification of the vesicles with carboxylic acids (SA) and polyethylene glycol (PEG) chains
          * Bone marrow-directing drug delivery material (Intravenous infusion)
          * Site-specific drug delivery system targeting bone marrow for various drugs
          * High blood compatibility and biodegradability

          Possible Applications:
          Drug delivery material which may be used in various applications such as diagnosis, treatment of prevention of diseases of bone, bone marrow, cartilage, and joint

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        • Apoptosis-Inducing Peptide for Cancer Gene TherapyApoptosis-Inducing Peptide for Cancer Gene Therapy
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            Description

            Provided is a multifunctional peptide which can induce apoptosis, increase of transfection efficiency with non-viral vectors, and increase of deposition of other proteins in extracellular matrix and therefore resulting in high local concentration of target proteins. The combination of these functions is ideal for improvement of apoptosis-based therapies for cancers. Currently, the inventor has been developing the combinational use of the proposed peptide and Fas-ligand (FasL) for gene therapy of oral and neck tumors. In vivo study using model mice with explanted tumors has shown that this peptide fragment can substantially improve the efficiency of cancer gene therapy using FasL. In fact, some tumors treated by the fused protein of FasL and this peptide completely disappeared.

            Features and Advantages
            * E3 and C1 domains of Del1, extracellular matrix (ECM) protein
            - Proteins fused to the E3 and C1 domains are deposited in the ECM
            - E3 also increases the endocytosis of transfected genes
            - E3 induces apoptosis at high concentration
            * Fusion protein of FasL and E3C1 can be used for improved cancer gene therapy
            - Local injection of the fusion protein using non-viral vectors
            - Injected protein is locally concentrated and retained due to its deposition activity
            - Safer and more economical than therapy by viral vector

            Possible Applications
            * Cancer gene therapy

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          • Single-stranded DNA Catenation EnzymeSingle-stranded DNA Catenation Enzyme
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              Description

              Provided is a novel method for single-stranded DNA (ssDNA) catenation, comprising ssDNA cutting and binding reactions. There is an increasing interest in methods for manipulating ssDNA, with the advance of genetic engineering techniques. However, only a limited number of techniques have been reported for manipulating ssDNA, especially for binding. It can be anticipated that this novel method will contribute to the elucidation of the generation of genetic disorder and new drug discovery.

              Features and Advantages
              EVL (human Ena/Vasp-like protein): ssDNA catenation enzyme
              * Stability of EVL: Storable at -80 degrees C
                -Stable for 1-2 years at deepfreeze (30% glycerol stock); capable of refreezing and rethawing
              * Preparation of EVL: Mass expression and recombinant purification using E.coli
                -Purifiable as His-Tag fusion protein (4-step purification)
              * Quality control of EVL: Electrophoresis
              * Activity confirmation of catenation: Electrophoresis and electronmicroscopy
              * Regulation of the efficiency of catenation
                -Efficiency is increased with addition of TopoI (10%->50%)
                -Efficiency is controllable with Mg2+ concentration

              Possible Applications:
              * Reagent for genetic engineering: ssDNA catenation reagent, molecular size marker
              * Screening tool: screening for the treatment of genetic disorder
              * DNA therapy: treatment using ssDNA

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            • MetadicholMetadichol
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                Eminent geneticist and cell biologist E.B. Wilson stated, "The key to every biological problem must finally be
                sought in the cell."
                Metadichol®, the lead product of NanoRx Inc, is a water-soluble form of Policosanol (average particle size is less than 60 nm) that in a clinical study on metabolic syndrome patients has shown some remarkable properties. In the 60-week study it showed improvements in Blood pressure, HDL, fasting blood glucose and insulin levels, kidney and liver function.
                US and international patents were published in August 2010. (U.S patent Application No 12/691706). A second patent appeared on Feb 2012 (U.S patent app No 13/211,132), which exploits the same principle by making changes in Octacosanol composition to affect other interesting biomarkers. In addition, national entry patents have been filed in EU, Japan, China India and other countries.
                Nanorx Inc has developed 4 products, each one of which has some unique properties and each can be obtained from the same source, i.e. sugar cane or rice wax, thus enabling economics of production scalability and ability to respond to demand. The company is looking for domestic and international licensing partners to commercialize Metadichol® as a nutraceutical or pharmaceutical product.

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              • MAPEMAPE
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                  Description

                  A self sustaining source of energy that uses Magnetic Acceleration Propulsion Energy to create easy, reliable energy. I invented the MAPE in 7th grade and wish to give it a go. Please, contact me for more information. I have no Patents, etc. So if one wishes for a further explanation, I will gladly speak. I am looking for fellow inventors to join this cause, as well as investors to help fund. I wish to eliminate the current fuel industry and replace it with a cleaner, more reliable form of energy for all the world to use. Please, contact me at nikolaskeosaia@yahoo.com for more information. Thank you.

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                • New sources of energyNew sources of energy
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                    Description

                    new energy sources.
                    Use gravity (gravity), geothermal effect (warm ground), solar energy, power flows of rivers, the wind.
                    Maximum security.
                    For the staff, (for maintenance), environment (ecology), for the state (strategic security).
                    High efficiency. High power (from 1KVt to 1000 MVt and more). Universal application.
                    The minimum cost price of electricity.

                    1 - power converter steam (air), low pressure. Patent RF 2215183.
                    When small amounts has enormous potential power. No competition. Able to replace nuclear
                    power, hydropower, thermal power plants.

                    2 - inverter air flow (of water). Patent RF 2219369.
                    more efficient than traditional turbines. When used in water can replace the hydroelectric power station.

                    3 - know-how. Super battery. The new principle of accumulation of electricity.
                    Able to replace hundreds of conventional batteries

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