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You selected inventions in category Biology

  • Nanopatch sensor in cell membraneNanopatch sensor in cell membrane
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      The present technology provides a real-time novel cellular platform which may be used for detecting occurrence of cellular biochemical processes that involve membrane perturbation or take place at membrane surfaces. The cellular platform i.e a chemical construct (nanopatch) comprising polydiacetylenes and lipids may be assembled within the cell membrane of living cells, and that said construct retains its ability to undergo its characteristic chromatic (red to blue) transition and associated fluorescent emission in live cells in response to various perturbing agents and conditions. It has further been unexpectedly found that following insertion of said construct into the cell membrane of living cells, the viability of said cells is retained for a period of time that is sufficient to permit the use of said cells as a system for detecting and/or quantifying biochemical events that lead to structural and morphological changes in the cell membrane and/or other processes occurring at the surface of the cell membrane.
      In contrast to existing cellular assays which, in general, detect specific chemical species or pathways within the cell, the cells and methods of the present invention are sensitive to any stimuli that induce functional and morphological modification of the cell membrane. A key feature of the claimed invention is the fact that following introduction of the nanopatches into the cell membrane, the target cells remain alive (and therefore useful for real-time investigations of processes in viable cells) for periods of at least four hours.

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    • A New Method for Cheaper and Longer Lasting BioimplantsA New Method for Cheaper and Longer Lasting...
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        The technology is therefore aimed at addressing the various shortcomings associated with the existing implants thereby improving implant making processes. The present technology provides novel titanium based composite bioactive (biocompatible) material useful as prosthetic implant as against conventional processes where-in the bioactive phases, usually calcium phosphatic chemicals, is present on the surface of the bioinert Ti. The novel titanium based implant allows tissue growth deep into the composite as against the conventional Ti based surface-coated implants which enable only surface integration because of the presence of bioactive phase only on the surface allowing tissue growth only at the surface of the implant, limited to the thickness of the bioactive coated phase. The implant when implanted in place of a damaged bone, can locate itself securely at the implantation and enables through integration with the healthy bone, thus completely avoiding any possibility of the implant getting loosened. The Ti based biocompatible material is capable of extracting out biological HA from the body fluids hence it substitutes the need of synthetic HA. Titanium oxide and certain calcium phosphatic and chemicals, which get formed in the present invention, have the potential to precipitate out biological HA from body fluids. This fact is proved by immersing the implant in SBF (Simulated Body Fluids). In biological HA the sizes of individual particles are more uniform. In the context of longevity of the implant, it is better to have more amount of biological HA in the implant and reduce the amount of synthetic HATitanium base powders are mixed with powder precursors of calcium and phosphorus, blended, milled and compacted. These compacts when calcined at 600-1200°C under various atmospheres form in situ calcium-phosphatic bioactive phases distributed uniformly in bioinert titanium phases. The calcined compacts are crushed, compacted to shape and vacuum sintered at 1000-1250°C to obtain appropriate interconnected porosity and strength. Immersion of the biocomposite in simulated body fluids, led to precipitation of bioactive phases like calcium hydroxyapatite, tricalcium phosphate, sodium calcium phosphate and calcium hydrogen phosphates on the surface, indicating biocompatibility of the implantable material having required interconnected porosity for facilitating tissue growth. The composite material thus developed by such process is noncytotoxic, has adequate corrosion properties, mechanical strength and can be used for orthopedic and other implants.The bioactive phases are not directly added to Ti, but added in the form of salts of calcium and phosphorus which during processing, convert into different bioactive phases within the bio inert Ti in situ - thereby ensuring proximity of the bioactive phases thus formed with the Ti phase. In vitro-cytotoxicity tests were performed and it was confirmed that the biocomposite produced by the process of this technology is non- cytotoxic.

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      • Rapid test for Multidrug Resistant InfectionsRapid test for Multidrug Resistant Infections
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          This technology suggests a rapid testing and profiling kit for multidrug resistance bacteria and for enabling real time monitoring so as to save lives, minimize expenses and reduce spread of drug resistant infections. The invention provides a solid support for bacterial culture of the type having an absorbent testing surface for solid phase bacteriological testing, comprising a plurality of intersecting lines of antibacterial compositions subdividing the surface into a multiplicity of individual test areas, antibacterial compositions serving as an effective barrier for preventing interference between adjacent test areas.
          A system based on using a selected enzyme as a marker for determining the sensitivity of an infection to antibacterial drugs and for identifying drug resistant strains. The system consists of two kits. The first kit detects the presence of a multidrug resistant bacterial infection and informs which, if any, of the beta-lactam antibiotics of choice can be used. If other antibacterial drugs are considered a second test kit provides the sensitivity profile needed for evidence-based treatment. It also includes the option of rapid identification of the infecting agent. Both kits are designed for direct point-of-care testing. Results of the first can be ready in less than 20 minutes; results of the second, in less than 3 hours.

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        • ODSTM-1 in-situ dyne surface tension wettability measurement at fast moving plastic films and foilsODSTM-1 in-situ dyne surface tension wettability...
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            Method and device for online tough less surface tension control ODSTM-1 in DE19543289 by which the fast moving substrate through the measuring gap. It is transmitted with a chromatic beam and spectral selected light source were two optical channels are displaced and polarized by 90 degree to each other. Both optical axles are precise and motor driven shift able in certain angles from 25 up to 65 degree. The spectral light photons, transmission, extinction, absorption grades are detectable by two optical CCD imagine vision devices, integrated into the sensor case on other side of the substrate.

            Material condition
            At high speed moving substrates means plastic foil, flexible, film, laminate, coating, bonding, labeling, co-extrusion, BOPP, LDPE, LLDPE, HDPE, MDPE, MAS, MOH, MEV, PET, FEP, PP, PE, PS, PO, EVA, PTFE, PVC, PTFE, Vinyl, Polyester, wrapping, self-adhesive tape, high strength, cross laminated, adhesive coated films, reflective or magnetic sheeting, automotive tape products, inkjet, heat sealing, security, pressure sensitive tapes for entertainment or building industry, masking or printable plastic films, polycarbonate, acrylic, extruded or polished cellulose, optical grade polycarbonate, sheets for IR or laser protection, filter sheet, imaging, capacitors, bi-oriented polystyrene sheet, multilayer co-extruded film, PVC, PETG, polyimide, tear tapes for aerospace, inner covering in aircrafts, automotive, medical, bioengineering, renewable, agriculture, marine, household, commercial, domestic, construction, high-porous membranes for uses in alkaline lithium ion batteries, fuel cells, filtration equipment. By gauges 10-500 microns, fabric widths up to 10000 mm and web speeds up to 18 m/s.

            Optical properties and quality control
            Material specific wavelength selection 1200 up to 1800 nm, material specific finger prints, molecular spectral properties, transmission, optical angle scanning, ultra low level stray scatter light detection, polarization, slot diaphragms, transverse displacements control the light beams along and far from the optical X and Y axes. The results are extreme scattering, diffraction, NIR, IR, stray light photons generation into the layer areas at both sides of the moving substrate.

            Their reflected and transmitted light intensities enables the determination by defined formulas and data matrixes the surface tension values in ranges from 28 up to 62 mN/m or dynes which are direct associate and bond to real static values for statistical quality control (SQC).

            And this entirely independent of the material specific influences as like surface consistency, thickness, density, weight, opacity, coherence, filling, stretching, shrinkage, structure, co-extrusion, polar grouping, temperature, intrinsic motivation or viscosity, hydrophobia, hydrophilic molecules, hydrogen, moisture, water steam proof, bi-layer, polymer, patterning, nanostructure, irradiation, isotopic, catalyst, coherence, absorption, photo mask effect, radiation, resonance bands, Raman, electron spectroscopy for chemical analysis ASTM or ATR method, property, acoustic photonic liquid effects, pre material treatment as corona, plasma jet, flam treatment.

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          • Full Color 3D Solid Image ScannerFull Color 3D Solid Image Scanner
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              This novel invention uses polishing techniques to slowly polish or abrade away stepped layers of an object to be scanned. The scanned object is cast into a contrasting resin which provides a dark (or light) background for photography. The layers are digitally photographed stepwise to produce a record of internal structures. 2D digital images are reassembled using computer and software methods to produce exquisitely fine detailed 3D digital models of the solid internal structures in full color.

              Because polishing can be controlled tightly, layers may be removed in the in the nanometer realm. This implies the limiting barrier in resolution is the pixel size of the recording camera. These cameras pixels are getting denser and denser as technology progresses which only improves the performance of the 3D scanner.

              The multi-spectral light used to illuminate the image plane can be used to identify the chemical components of the object. Therefore the 3D scanner has a spectrometer function as well.

              The Full Color 3D Solid Image Scanner would be important in the semiconductor industry. Most importantly 1st article scanning semiconductor devices to determine as manufactured state vs. as designed.

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            • Interferometric method for recoding of dynamic volume changes in living and non-living objectsInterferometric method for recoding of dynamic...
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                The invention describes a method and apparatus assigned primarily for volume dynamics investigation of physiologically active samples such as higher or lower plants, algae, photosynthetic bacteria, or parts of these organisms. The investigation is aimed at qualitative and quantitative determination of volume change dynamics of samples in a micrometric range, particularly in dependence on their photosynthetic activity in vivo. Application of this invention in other areas, e.g. precise mechanics and engineering, is expected.

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              • Optimized Expression of IL-12 Cytokine FamilyOptimized Expression of IL-12 Cytokine Family
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                  The IL-12 family of cytokines (IL-12, IL-23, and IL-27) has an important role in inflammation and autoimmune diseases. IL-12 is produced by macrophages and dendritic cells in response to certain bacterial and parasitic infections and is a powerful inducer of IFN-gamma production. IL-23 is proposed to stimulate a subset of T cells to produce IL-17, which in turn induce the production of proinflammatory cytokines that lead to a protective response during infection. IL-27 appears to have duel functions as an initiator of TH1-type (cellular immunity) immune responses and as an attenuator of immune/inflammatory responses.

                  The present inventions provide methods for improved expression of multimeric proteins by engineering different ratios of the subunit expression units in a cell or upon expression from a multi-promoter plasmid having different strength promoters. The inventors have improved the levels and efficiency of expression of the IL-12 family of cytokines, which includes IL-12, IL-23, and IL-27, by adjusting the transcription and translation of the alpha and beta subunits that comprise the heterodimeric proteins. Optimal ratios of expression for the two (2) subunits were determined for IL-12, IL-23, and IL-27.

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